Journal:

Article Title: Crosstalk between the Ras2p-controlled Mitogen-activated Protein Kinase and cAMP Pathways during Invasive Growth of Saccharomyces cerevisiae

doi:

Figure Lengend Snippet: Expression of different RAS gene constructs in a ras2 mutant strain. (A) Shown is invasive growth of yeast strain YHUM86 (ras2) containing YCplac22 (vector), YCplac22-RAS2 (RAS2), YCplac22-RAS2Val19 (RAS2V19), YEplac112-RAS2 (2 μm RAS2), pRS314-RAS2ΔC (RAS2ΔC), pRS314-R2N-R1C (R2N-R1C), YEplac112-RAS1 (RAS1 2 μm) or YCplac22-R2p-R1orf (R2p-R1orf). Plates were incubated for 4 d and photographed before (Total growth) and after (Invasive growth) washing cells of the agar surface. (B) Expression levels of Ras proteins was determined in strains described in A by Western blot analysis using monoclonal anti-H-Ras antibody (259) that cross-reacts equally well with either Ras1p or Ras2p (upper panel, short exposure; lower panel, long exposure). (C) Autoradiogram showing steady-state levels of RAS1 and RAS2 mRNA in strains described in A. ACT1 gene expression was used as internal standard. (D) FG(Ty1)::lacZ expression levels. β-Galactosidase activity was measured on strains described in A. Units are nanomoles per minute per milligram. Bars depict means of three independent measurements. White bars indicate RAS2-independent expression of FG(Ty1)::lacZ.

Article Snippet: Ras proteins were detected using ECL technology (Amersham, Buckinghamshire, United Kingdom) after incubation of membranes with the rat monoclonal anti-H-Ras antibody (259) (Santa Cruz Biotechnology, Santa Cruz, CA) and a peroxidase-coupled goat anti-rat IgG secondary antibody (Dianova, Hamburg, Germany).

Techniques: Expressing, Construct, Mutagenesis, Plasmid Preparation, Incubation, Western Blot, Activity Assay

Journal: PLoS ONE

Article Title: Distribution and Characterization of Progenitor Cells within the Human Filum Terminale

doi: 10.1371/journal.pone.0027393

Figure Lengend Snippet: BrdU-positive (green) and Sox2-positive (red) cells in neurosphere from human FT (Insert in A). (B) Nestin-positive cells (green) after 28 days with nuclear marker Hoechst (blue). (C) GFAP-positive cells with astroglial shape (red) and (D) One neuronal (β-III-tubulin) cell as it appears after 11 days of differentiation (blue = DAPI). (E) Expansion of cells without PDGF-BB. (F) Cells expanded in culture medium exposed to PDGF-BB for 8 days, GFAP (red), β-III tubulin (green) and nuclear marker Hoechst (blue). Bars: A and B = 10 μm, Insert = 25 μm, C and D = 5 μm and E−F = 20 μm.

Article Snippet: Preparations were incubated with the primary antibodies; rabbit-anti-GFAP 1∶1000 (DAKO), mouse anti-h-Nestin 1∶1000(R&D Systems), mouse anti-Tubulin β-III 1∶50 (Chemicon) for 24 h at 4° C, rinsed in PBS and subsequently incubated with species-specific secondary antibodies diluted in PBS; Alexa 488 donkey anti-mouse 1∶500 (Molecular Probes/Invitrogen), Cy3 donkey anti-rabbit 1∶1000 (Jackson Immunoresearch).

Techniques: Marker

Journal: PLoS ONE

Article Title: Distribution and Characterization of Progenitor Cells within the Human Filum Terminale

doi: 10.1371/journal.pone.0027393

Figure Lengend Snippet: (A) Longitudinal section of human FT stained with haematoxylin-eosin. The human FT mostly consists of astrocytes and ependymal cells (see arrows) (B–D). Transverse sections of rat FT stepwise from conus (B) and further caudally (C and D) the central canal is prominent in the middle of the rat film. Rounded brown structures in the perperiphery represent nerve roots. (E and F). Longitudinal sections of the rat FT labelled with a nestin antibody (red) and a nuclear marker DAPI (blue). (F) Higher magnification of boxed area in (E). Bars: A and E = 40 µm, B and C = 100 µm, F = 5 µm. Asterix = central canal.

Article Snippet: Preparations were incubated with the primary antibodies; rabbit-anti-GFAP 1∶1000 (DAKO), mouse anti-h-Nestin 1∶1000(R&D Systems), mouse anti-Tubulin β-III 1∶50 (Chemicon) for 24 h at 4° C, rinsed in PBS and subsequently incubated with species-specific secondary antibodies diluted in PBS; Alexa 488 donkey anti-mouse 1∶500 (Molecular Probes/Invitrogen), Cy3 donkey anti-rabbit 1∶1000 (Jackson Immunoresearch).

Techniques: Staining, Marker